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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF1795 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects feline IL-5 in direct ELISAs and Western blots. In direct ELISAs, approximately 80% cross-reactivity with recombinant bovine IL-5 and recombinant equine IL-5 is observed, approximately 50% cross-reactivity with recombinant porcine IL-5 and recombinant canine IL-5 is observed, approximately 10% cross-reactivity with recombininat human IL-5 is observed, and less than 1% cross-reactivity with recombinant rat IL-5, recombinant mouse IL-5, and recombinant rhesus monkey IL-5 is observed.
- Reactivity
- Feline
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-5 and Neutralization by Feline IL-5 Antibody. Recombinant Feline IL-5 (Catalog # 1795-FL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL-5 (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1795). The ND50 is typically 0.5-2.0 µg/mL.
