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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF2470 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-5 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects equine IL-5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant bovine IL-5 and recombinant feline IL-5 is observed, 30% cross-reactivity with recombinant mouse IL-5 and recombinant canine IL-5 is observed, 20% cross-reactivity with recombinant rhesus macaque IL-5 is observed, and less than 10% cross-reactivity with recombinant rat IL-5 and recombinant porcine IL-5 is observed.
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Treating insect-bite hypersensitivity in horses with active vaccination against IL-5.
Fettelschoss-Gabriel A, Fettelschoss V, Thoms F, Giese C, Daniel M, Olomski F, Kamarachev J, Birkmann K, Bühler M, Kummer M, Zeltins A, Marti E, Kündig TM, Bachmann MF
The Journal of allergy and clinical immunology 2018 Oct;142(4):1194-1205.e3
The Journal of allergy and clinical immunology 2018 Oct;142(4):1194-1205.e3
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-5 and Neutralization by Equine IL-5 Antibody. Recombinant Equine IL-5 (Catalog # 2470-EL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Equine IL-5 (50 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Equine IL-5 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2470). The ND50 is typically 0.5-2.0 µg/mL.
