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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF3137 - Provider product page
- Provider
- R&D Systems
- Product name
- Porcine IL-5 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects porcine IL-5 in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross-reactivity with recombinant canine IL-5 and less than 10% cross-reactivity with recombinant human IL-5, recombinant mouse IL-5, and recombinant rat IL-5 is observed.
- Reactivity
- Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9MYM5- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-5 in Porcine PBMCs. IL-5 was detected in immersion fixed porcine peripheral blood mononuclear cells treated with calcium ionomycin and PMA using Goat Anti-Porcine IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3137) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-5 and Neutralization by Porcine IL-5 Antibody. Recombinant Porcine IL-5 (Catalog # 3137-PL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-5 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Porcine IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3137). The ND50 is typically <5 µg/mL.
