Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF1964 - Provider product page
- Provider
- R&D Systems
- Product name
- Canine IL-5 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects canine canine IL-5 in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant bovine IL-5, recombinant equine IL-5, and recombinant feline IL-5 is observed and less than 5% cross-reactivity with recombinant rat IL-5, recombinant mouse IL-5, and recombinant human IL-5 is observed.
- Reactivity
- Canine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q95J76
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-5 in Canine PBMCs. IL-5 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Goat Anti-Canine IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1964) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-5 and Neutralization by Canine IL-5 Antibody. Recombinant Canine IL-5 (Catalog # 1964-CL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-5 (30 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Canine IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1964). The ND50 is typically 0.75-3.0 µg/mL.