MA5-23690

antibody from Invitrogen Antibodies

Targeting: IL5 EDF, IL-5, TRF

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-23690

Provider

Invitrogen Antibodies

Product name

IL-5 Monoclonal Antibody (TRFK5)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

In sandwich ELISAs, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, recombinant mouse (rm) IL-5, rhIL-5, rmIL-3, or rmGM-CSF is observed.

Antibody clone number

TRFK5

Concentration

0.5 mg/mL

References

Submitted references

A mechanism for the induction of type 2 immune responses by a protease allergen in the genital tract.

Oh JE, Oh DS, Jung HE, Lee HK
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 14;114(7):E1188-E1195

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Role of arginase 1 from myeloid cells in th2-dominated lung inflammation.

Barron L, Smith AM, El Kasmi KC, Qualls JE, Huang X, Cheever A, Borthwick LA, Wilson MS, Murray PJ, Wynn TA
PloS one 2013;8(4):e61961

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Influence of mucosal adjuvants on antigen passage and CD4+ T cell activation during the primary response to airborne allergen.

Wikstrom ME, Batanero E, Smith M, Thomas JA, von Garnier C, Holt PG, Stumbles PA
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 15;177(2):913-24

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of human peripheral blood monocytes treated with 50 ng/mL PMA and 500 ng/mL Calcium Ionomycin for 6 hours were stained with human/mouse IL-5 Monoclonal Antibody (Product # MA5-23690) followed by Phycoerythrin-conjugated Anti-Rat IgG F (ab')2 Secondary Antibody and human CD3e APC-conjugated Monoclonal Antibody. Quadrant markers were set based on control antibody staining. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.