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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- IC605F - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-5 Fluorescein-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IL-5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL-5 is observed.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
P05113- Isotype
- IgG
- Antibody clone number
- 9906
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references IL-33 induces IL-9 production in human CD4+ T cells and basophils.
Blom L, Poulsen BC, Jensen BM, Hansen A, Poulsen LK
PloS one 2011;6(7):e21695
PloS one 2011;6(7):e21695
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of IL-5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin were stained with Mouse Anti-Human IL-5 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC605F) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
