18171-1-AP

antibody from Invitrogen Antibodies

Targeting: FGF5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

18171-1-AP

Provider

Invitrogen Antibodies

Product name

FGF5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LSAWAHGEK RLAPKGQPGP AATDRNPRGS SSRQSSSSAM SSSSASSSPA ASLGSQGSGL EQSSFQWSPS GRRTGSLYCR VGIGFHLQIY PDGKVNGSHE ANMLSVLEIF AVSQGIVGIR GVFSNKFLAM SKKGKLHASA KFTDDCKFRE RFQENSYNTY ASAIHRTEKT GREWYVALNK RGKAKRGCSP RVKPQHISTH FLPRFKQSEQ PELSFTVTVP EKKKPPSPIK PKIPLSAPRK NTNSVKYRLK FRFG (16-268 aa encoded by BC074858)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.21 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18171-1-AP (FGF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18171-1-AP (FGF5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 18171-1-AP ( FGF5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 18171-1-AP (FGF5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NIH/3T3 cells using 18171-1-AP (FGF5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta tissue slide using 18171-1-AP (FGF5 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta tissue slide using 18171-1-AP (FGF5 Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human stomach tissue slide using 18171-1-AP ( FGF5 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human stomach tissue slide using 18171-1-AP ( FGF5 Antibody) at dilution of 1:200 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-FGF5 (IP:18171-1-AP, 4ug; Detection:18171-1-AP 1:500) with A375 cells lysate 3200ug.