720149

antibody from Invitrogen Antibodies

Targeting: FGF10

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

720149

Provider

Invitrogen Antibodies

Product name

FGF10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa (Condition Media) (Lane 2), HEK-293 (Lane 3), HEK-293 (Condition Media) (Lane 4), A549 (Lane 5), A549 (Condition Media) (Lane 6), Huvec (Lane 7) and Huvec (Condition Media) (Lane 8). The blots were probed with Anti-FGF10 Rabbit Polyclonal Antibody (Product # 720149, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 36 kDa band corresponding to FGF10 was observed in the condition media of the cell lines tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).