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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- 500-P151G-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FGF10 Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS.
- Concentration
- 0.1-1.0 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human FGF-10 by Western Blot analysis FGF10 Polyclonal Antibody (Product # 500-P151G-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human FGF-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human FGF-10 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of FGF10 Polyclonal Antibody (Product # 500-P151G-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech FGF10 Polyclonal Antibody, Biotin (Product # 500-P151GBT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human FGF-10.
