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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunoprecipitation [1]
- Immunohistochemistry [5]
- Flow cytometry [1]
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- Product number
- GTX83887 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX83887, RRID:AB_10732166
- Product name
- PIM2 antibody [5D5]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Anti-PIM2 mouse monoclonal antibody (GTX83887) immunofluorescent staining of COS7 cells transiently transfected with PIM2
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- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-PIM2 mouse monoclonal antibody (GTX83887).
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- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-PIM2 mouse monoclonal antibody (GTX83887).
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of PIM2 by using TrueMab monoclonal anti-PIM2 antibodies (Negative control: IP without adding anti-PIM2 antibody). For each experiment, 500ul of DDK tagged PIM2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PIM2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-RC201933 mouse monoclonal antibody. (GTX83887, Dilution 1:50)
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- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-RC201933 mouse monoclonal antibody. (GTX83887, Dilution 1:50)
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- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue using anti-RC201933 mouse monoclonal antibody. (GTX83887, Dilution 1:50)
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- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-RC201933 mouse monoclonal antibody. (GTX83887, Dilution 1:50)
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- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded prostate tissue using anti-RC201933 mouse monoclonal antibody. (GTX83887, Dilution 1:50)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY PIM2
