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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-22315 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PIM2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, mouse testis.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PIM2 Polyclonal Antibody (Product # PA5-22315). Sample (30 µg of whole cell lysate). Lane A: 293T. 10% SDS PAGE. PIM2 Polyclonal Antibody (Product # PA5-22315) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PIM2 Polyclonal Antibody detects PIM2 protein by Western blot analysis. A. 50 µg mouse testis lysate/extract.12 % SDS-PAGE. PIM2 Polyclonal Antibody (Product # PA5-22315) dilution: 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PIM2 Polyclonal Antibody (Product # PA5-22315) and a 38 kDa band corresponding to PIM2 was observed in Mouse Spleen, Mouse Thymus and Mouse Heart except Mouse Liver which is reported to be low. Tissue extracts (30 µg lysate) of Mouse Spleen (Lane 1), Mouse Thymus (Lane 2), Mouse Heart (Lane 3) and Mouse Liver (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using PIM2 (Product # PA5-22315) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
