Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF959 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Erythropoietin/EPO Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat Erythropoietin/EPO in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Thymopoietin (Tpo) is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P07321
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Zinc Supplementation Stimulates Red Blood Cell Formation in Rats.
Suppression of hepcidin during anemia requires erythropoietic activity.
Chen YH, Feng HL, Jeng SS
International journal of molecular sciences 2018 Sep 18;19(9)
International journal of molecular sciences 2018 Sep 18;19(9)
Suppression of hepcidin during anemia requires erythropoietic activity.
Pak M, Lopez MA, Gabayan V, Ganz T, Rivera S
Blood 2006 Dec 1;108(12):3730-5
Blood 2006 Dec 1;108(12):3730-5
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by Erythropoietin/EPO and Neutralization by Mouse Erythropoietin/EPO Antibody. Recombinant Mouse Erythropoietin/EPO (Catalog # 959-ME) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Erythropoietin/EPO (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Erythropoietin/EPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF959). The ND50 is typically 0.25-1.25 µg/mL.