LS-C18929

antibody from LSBio

Targeting: JAG1 AGS, AHD, AWS, CD339, HJ1, JAGL1

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C18929

Provider

LSBio

Proper citation

LifeSpan Cat#LS-C18929, RRID:AB_2128245

Product name

JAG1 / Jagged 1 Antibody (N-Terminus) LS-C18929

Antibody type

Polyclonal

Description

Protein A purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-Jagged-1 Antibody - Western Blot. Western blot of Protein A purified anti-Jagged-1 antibody shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only. The band at ~134 kD in lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown. Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG. Exposure time was 1 min. Predicted molecular weight is 134 kD.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry - Jagged-1 Antibody.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry - Jagged-1 Antibody.