LS-C291044

antibody from LSBio

Targeting: JAG1 AGS, AHD, AWS, CD339, HJ1, JAGL1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

LS-C291044

Provider

LSBio

Product name

Anti-JAG1 / Jagged 1 Antibody (aa1168-1218) LS-C291044

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse, Rat, Chicken/Avian, Xenopus, Zebrafish

Host

Rabbit

Vial size

100µl

Storage

Store at 2-8°C for up to 1 year.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human Jagged1 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HepG2 cells. Antibodies: Affinity purified rabbit anti-Jagged1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human Jagged1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HepG2 cells. Antibodies: Affinity purified rabbit anti-Jagged1 antibody used for IP at 6 ug per reaction. Jagged1 was also immunoprecipitated by rabbit anti-Jagged1 antibody BL16002. For blotting immunoprecipitated Jagged1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.