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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Flow cytometry [2]
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- Product number
- MAB1277-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Jagged 1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, and rat Jagged 1. In direct ELISAs, no cross-reactivity with recombinant human Jagged 2 is observed. Stains human Jagged 1 transfectants but not the parental cell line or human Jagged 2 transfectants in flow cytometry.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P78504- Isotype
- IgG
- Antibody clone number
- 188331
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Diabetes impairs arterio-venous specification in engineered vascular tissues in a perivascular cell recruitment-dependent manner.
Jagged1 Instructs Macrophage Differentiation in Leprosy.
The CD46-Jagged1 interaction is critical for human TH1 immunity.
Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma.
Antiparallel segregation of notch components in the immunological synapse directs reciprocal signaling in allogeneic Th:DC conjugates.
Differential induction of Th1-prone immunity by human dendritic cells activated with Sporothrix schenckii of cutaneous and visceral origins to determine their different virulence.
Altalhi W, Sun X, Sivak JM, Husain M, Nunes SS
Biomaterials 2017 Mar;119:23-32
Biomaterials 2017 Mar;119:23-32
Jagged1 Instructs Macrophage Differentiation in Leprosy.
Kibbie J, Teles RM, Wang Z, Hong P, Montoya D, Krutzik S, Lee S, Kwon O, Modlin RL, Cruz D
PLoS pathogens 2016 Aug;12(8):e1005808
PLoS pathogens 2016 Aug;12(8):e1005808
The CD46-Jagged1 interaction is critical for human TH1 immunity.
Le Friec G, Sheppard D, Whiteman P, Karsten CM, Shamoun SA, Laing A, Bugeon L, Dallman MJ, Melchionna T, Chillakuri C, Smith RA, Drouet C, Couzi L, Fremeaux-Bacchi V, Köhl J, Waddington SN, McDonnell JM, Baker A, Handford PA, Lea SM, Kemper C
Nature immunology 2012 Dec;13(12):1213-21
Nature immunology 2012 Dec;13(12):1213-21
Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma.
Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A
The Journal of allergy and clinical immunology 2009 Jan;123(1):239-48
The Journal of allergy and clinical immunology 2009 Jan;123(1):239-48
Antiparallel segregation of notch components in the immunological synapse directs reciprocal signaling in allogeneic Th:DC conjugates.
Luty WH, Rodeberg D, Parness J, Vyas YM
Journal of immunology (Baltimore, Md. : 1950) 2007 Jul 15;179(2):819-29
Journal of immunology (Baltimore, Md. : 1950) 2007 Jul 15;179(2):819-29
Differential induction of Th1-prone immunity by human dendritic cells activated with Sporothrix schenckii of cutaneous and visceral origins to determine their different virulence.
Uenotsuchi T, Takeuchi S, Matsuda T, Urabe K, Koga T, Uchi H, Nakahara T, Fukagawa S, Kawasaki M, Kajiwara H, Yoshida S, Moroi Y, Furue M
International immunology 2006 Dec;18(12):1637-46
International immunology 2006 Dec;18(12):1637-46
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Jagged 1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endo-thelioma cell line was stained with Mouse Anti-Human Jagged 1 Monoclonal Antibody (Catalog # MAB1277, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Jagged 1 in Rat Cortical Stem Cells by Flow Cytometry. Rat cortical stem cells were stained with Mouse Anti-Human Jagged 1 Monoclonal Antibody (Catalog # MAB1277, filled histogram) or isotype control antibody (Catalog # MAB0041, open histo-gram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
