Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB9954 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9954, RRID:AB_1676561
- Product name
- JAG1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of JAG1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Immunohistological localization of Notch receptors and their ligands Delta and Jagged in synovial tissues of rheumatoid arthritis.
Complementation of human papillomavirus type 16 E6 and E7 by Jagged1-specific Notch1-phosphatidylinositol 3-kinase signaling involves pleiotropic oncogenic functions independent of CBF1;Su(H);Lag-1 activation.
Crosstalk between Jagged1 and GDNF/Ret/GFRalpha1 signalling regulates ureteric budding and branching.
Yabe Y, Matsumoto T, Tsurumoto T, Shindo H
Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association 2005 Nov;10(6):589-94
Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association 2005 Nov;10(6):589-94
Complementation of human papillomavirus type 16 E6 and E7 by Jagged1-specific Notch1-phosphatidylinositol 3-kinase signaling involves pleiotropic oncogenic functions independent of CBF1;Su(H);Lag-1 activation.
Veeraraghavalu K, Subbaiah VK, Srivastava S, Chakrabarti O, Syal R, Krishna S
Journal of virology 2005 Jun;79(12):7889-98
Journal of virology 2005 Jun;79(12):7889-98
Crosstalk between Jagged1 and GDNF/Ret/GFRalpha1 signalling regulates ureteric budding and branching.
Kuure S, Sainio K, Vuolteenaho R, Ilves M, Wartiovaara K, Immonen T, Kvist J, Vainio S, Sariola H
Mechanisms of development 2005 Jun;122(6):765-80
Mechanisms of development 2005 Jun;122(6):765-80
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using JAG1 polyclonal antibody (Cat # PAB9954) shows detection of JAG1 protein in various whole cell lysates : human brain (Lane 1), human kidney (Lane 2), human liver (Lane 3), and mouse liver (Lane 5).Lane 4 contained sample buffer only.The band at ~134 KDa in lane 5 is believed to be JAG1 precursor.The identity of minor reactive bands is unknown.Each lane contains approximately 20 ug of lysate.Primary antibody was used at a 1 : 500 dilution.The membrane was washed and reacted with a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG.Exposure time was 1 min.Predicted molecular weight is 134 KDa.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence Microscopy using JAG1 polyclonal antibody (Cat # PAB9954) of human corneal epithelial cells.Primary antibody was used at a 1 : 500 dilution.The JAG1 (green staining) is local-ized to the cytoplasm and is consistent with reports in the literature.The nucleus is stained with Bis benzimide (blue) .Personal Communication. Aihua Ma, Univdersity of Cardiff.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human cervical cancer tissue (40X magnification) using JAG1 polyclonal antibody (Cat # PAB9954) . Tissue was fixed with formalin and embedded in paraffin.Hematoxylin was used to counter-stain cells. A 1 : 100 dilution of primary antibody was used.Personal Communication. Martin Kast Laboratory.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
