- PA5-72843 - Invitrogen Antibodies JAG1 antibody

Lustofin S, Kamińska A, Brzoskwinia M, Cyran J, Kotula-Balak M, Bilińska B, Hejmej A
International journal of molecular sciences 2022 Feb 18;23(4)

Gong M, Yan F, Yu L, Li F
Cell death & disease 2022 Aug 27;13(8):738

Kamińska A, Marek S, Pardyak L, Brzoskwinia M, Pawlicki P, Bilińska B, Hejmej A
Reproductive biology and endocrinology : RB&E 2020 Apr 16;18(1):30

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of JAG1 in human spleen tissue with Jagged1 Polyclonal Antibody (Product # PA5-72843) at 5 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence of JAG1 in human spleen tissue with Jagged1 Polyclonal Antibody (Product # PA5-72843) at 20 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: The effect of androgen receptor antagonists on mRNA and protein expression of Jag1 /JAG1, Dll1 /DLL1, and Dll4 /DLL4 in primary rat Sertoli cells. Cells were treated with 10 -8 M testosterone (T), 10 -4 M hydroxyflutamide (HF), HF + T, 10 -6 M bicalutamide (Bic), Bic + T, or vehicle (C) for 24 h. ( a , c , e ) Relative expression of mRNAs (RQ) was determined using real-time RT-PCR analysis. The expression values of the individual genes were normalized to the mean expression of the reference genes. ( b , d , f ) Western blot detection of the proteins. The relative level of studied protein was normalized to beta-actin. The protein levels within the control group were arbitrarily set at 1. The histograms are the quantitative representation of data (mean +- SD) of three independent experiments, each in triplicate. Significant differences from control values are denoted as * p < 0.05, ** p < 0.01, and *** p < 0.001. ( g ) Immunofluorescence analysis of JAG1, DLL1, and DLL4 expression. Scale bar = 10 um.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: The effect of androgen receptor antagonists or androgen receptor silencing on mRNA and protein expression of Jag1 /JAG1, Dll1 /DLL1, and Dll4 /DLL4 in TM4 Sertoli cell line. ( a - g ) Cells were treated with 10 -8 M testosterone (T), 10 -4 M hydroxyflutamide (HF), HF + T, 10 -6 M bicalutamide (Bic), Bic + T, or vehicle (C) for 24 h. ( h - n ) Cells were treated with transfection reagent alone (C), transfection reagent + 5 x 10 -8 M non-targeting siRNA (negative control, NT), transfection reagent + 5 x 10 -8 M AR siRNA (AR-Kd), or ZIP9 siRNA (ZIP9-Kd). After 24 h, 10 -8 M T or vehicle was added to the culture. ( a , c , e , h , j , l ) Relative expression of mRNAs (RQ) was determined using real-time RT-PCR analysis. The expression values of the individual genes were normalized to the mean expression of the reference genes. ( b , d , f , i , k , m ) Western blot detection of the proteins. The relative level of studied protein was normalized to beta-actin. The protein levels within the control group were arbitrarily set at 1. The histograms are the quantitative representation of data (mean +- SD) of three independent experiments, each in triplicate. Significant differences from control values are denoted as * p < 0.05, ** p < 0.01, and *** p < 0.001. ( g , n ) Immunofluorescence analysis of JAG1, DLL1, and DLL4 expression. Scale bar = 10 um.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: The effect of estrogen receptor antagonists on mRNA and protein expression of Jag1 /JAG1, Dll1 /DLL1, and Dll4 /DLL4 in primary rat Sertoli cells. Cells were treated with 10 -9 M 17beta-estradiol (E), 10 -6 M ICI 182,780 (ICI), ICI + E, 10 -8 M G15, G15 + E, or vehicle (C) for 24 h. ( a , c , e ) Relative expression of mRNAs (RQ) was determined using real-time RT-PCR analysis. The expression values of the individual genes were normalized to the mean expression of the reference genes. ( b , d , f ) Western blot detection of the proteins. The relative level of studied protein was normalized to beta-actin. The protein levels within the control group were arbitrarily set at 1. The histograms are the quantitative representation of data (mean +- SD) of three independent experiments, each in triplicate. Significant differences from control values are denoted as * p < 0.05, ** p < 0.01, and *** p < 0.001. ( g ) Immunofluorescence analysis of JAG1, DLL1, and DLL4 expression. Scale bar = 10 um.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: The effect of estrogen receptor antagonists or estrogen receptor silencing on mRNA and protein expression of Jag1 /JAG1, Dll1 /DLL1, and Dll4 /DLL4 in TM4 Sertoli cell line. ( a - g ) Cells were treated with 10 -9 M 17beta-estradiol (E), 10 -6 M ICI 182,780 (ICI), ICI + E, 10 -8 M G15, G15 + E, or vehicle (C) for 24 h. ( h - n ) Cells were treated with transfection reagent alone (C), transfection reagent + 5 x 10 -8 M non-targeting siRNA (negative control, NT), transfection reagent + 5 x 10 -8 M ERalpha siRNA (ERalpha -Kd), ERbeta siRNA (ERbeta -Kd), or GPER siRNA (GPER-Kd). After 24 h, 17beta-estradiol or vehicle was added to the culture. ( a , c , e , h , j , l ) Relative expression of mRNAs (RQ) was determined using real-time RT-PCR analysis. The expression values of the individual genes were normalized to the mean expression of the reference genes. ( b , d , f , i , k , m ) Western blot detection of the proteins. The relative level of studied protein was normalized to beta-actin. The protein levels within the control group were arbitrarily set at 1. The histograms are the quantitative representation of data (mean +- SD) of three independent experiments, each in triplicate. Significant differences from control values are denoted as * p < 0.05, ** p < 0.01, and *** p < 0.001. ( g , n ) Immunofluorescence analysis of JAG1, DLL1, and DLL4 expression. Scale bar = 10 um.

PA5-72843