Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB600-1161-0.25mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Jagged 1 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.25 mg
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Jagged 1 Antibody [NB600-1161] - Analysis of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only. The band at 134 kDa in lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown. Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG. Exposure time was 1 min. Predicted molecular weight is 134 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Jagged 1 Antibody [NB600-1161] - Human cervical cancer tissue (40X magnification) using NB600-1161 Protein A purified anti- Jagged-1 antibody. Tissue was fixed with formalin and embedded in paraffin. Hematoxylin was used to counter-stain cells. A 1:100 dilution of primary antibody was used.