Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF1277 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Jagged 1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Jagged 1 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant rat Jagged 1 and recombinant human Jagged 2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P78504
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references JAG1 overexpression contributes to Notch1 signaling and the migration of HTLV-1-transformed ATL cells.
IL-4-dependent Jagged1 expression/processing is associated with survival of chronic lymphocytic leukemia cells but not with Notch activation.
Evolution of Cancer Stem-like Cells in Endocrine-Resistant Metastatic Breast Cancers Is Mediated by Stromal Microvesicles.
Interleukin-6 Stimulates Defective Angiogenesis.
A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment.
Interleukin-6 as a therapeutic target in human ovarian cancer.
Identification of the surface-accessible, lineage-specific vascular proteome by two-dimensional peptide mapping.
Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling.
Bellon M, Moles R, Chaib-Mezrag H, Pancewicz J, Nicot C
Journal of hematology & oncology 2018 Sep 19;11(1):119
Journal of hematology & oncology 2018 Sep 19;11(1):119
IL-4-dependent Jagged1 expression/processing is associated with survival of chronic lymphocytic leukemia cells but not with Notch activation.
De Falco F, Del Papa B, Baldoni S, Sabatini R, Falzetti F, Di Ianni M, Martelli MP, Mezzasoma F, Pelullo M, Marconi P, Sportoletti P, Screpanti I, Rosati E
Cell death & disease 2018 Nov 26;9(12):1160
Cell death & disease 2018 Nov 26;9(12):1160
Evolution of Cancer Stem-like Cells in Endocrine-Resistant Metastatic Breast Cancers Is Mediated by Stromal Microvesicles.
Sansone P, Berishaj M, Rajasekhar VK, Ceccarelli C, Chang Q, Strillacci A, Savini C, Shapiro L, Bowman RL, Mastroleo C, De Carolis S, Daly L, Benito-Martin A, Perna F, Fabbri N, Healey JH, Spisni E, Cricca M, Lyden D, Bonafé M, Bromberg J
Cancer research 2017 Apr 15;77(8):1927-1941
Cancer research 2017 Apr 15;77(8):1927-1941
Interleukin-6 Stimulates Defective Angiogenesis.
Gopinathan G, Milagre C, Pearce OM, Reynolds LE, Hodivala-Dilke K, Leinster DA, Zhong H, Hollingsworth RE, Thompson R, Whiteford JR, Balkwill F
Cancer research 2015 Aug 1;75(15):3098-107
Cancer research 2015 Aug 1;75(15):3098-107
A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment.
Kulbe H, Chakravarty P, Leinster DA, Charles KA, Kwong J, Thompson RG, Coward JI, Schioppa T, Robinson SC, Gallagher WM, Galletta L, Australian Ovarian Cancer Study Group., Salako MA, Smyth JF, Hagemann T, Brennan DJ, Bowtell DD, Balkwill FR
Cancer research 2012 Jan 1;72(1):66-75
Cancer research 2012 Jan 1;72(1):66-75
Interleukin-6 as a therapeutic target in human ovarian cancer.
Coward J, Kulbe H, Chakravarty P, Leader D, Vassileva V, Leinster DA, Thompson R, Schioppa T, Nemeth J, Vermeulen J, Singh N, Avril N, Cummings J, Rexhepaj E, Jirström K, Gallagher WM, Brennan DJ, McNeish IA, Balkwill FR
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Sep 15;17(18):6083-96
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Sep 15;17(18):6083-96
Identification of the surface-accessible, lineage-specific vascular proteome by two-dimensional peptide mapping.
Roesli C, Mumprecht V, Neri D, Detmar M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Jun;22(6):1933-44
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Jun;22(6):1933-44
Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling.
Liotta F, Angeli R, Cosmi L, Filì L, Manuelli C, Frosali F, Mazzinghi B, Maggi L, Pasini A, Lisi V, Santarlasci V, Consoloni L, Angelotti ML, Romagnani P, Parronchi P, Krampera M, Maggi E, Romagnani S, Annunziato F
Stem cells (Dayton, Ohio) 2008 Jan;26(1):279-89
Stem cells (Dayton, Ohio) 2008 Jan;26(1):279-89
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Jagged 1 by Western Blot. Western blot shows lysates of Huh-7 human hepatoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1277) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Jagged 1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Jagged 1 in Human Kidney Cancer Tissue. Jagged 1 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1277) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Alkaline Phosphatase Production Induced by Jagged 1 and Neutralization by Human Jagged 1 Antibody. Recombinant Human Jagged 1 Fc Chimera (Catalog # 1277-JG) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human/Mouse/Rat BMP-2 (Catalog # 355-BM) in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human Jagged 1 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Jagged 1 Antibody (Catalog # AF1277). The ND50 is typically 1-5 µg/mL.