sc-8349

RAD51 antibody from Santa Cruz Biotechnology

BRCC5, FANCR, HsRad51, HsT16930, RAD51A, RECA

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for sc-8349

Antibody Data

Product number

sc-8349

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-8349, RRID:AB_2253533

Product name

Anti-RAD51

Provider product page

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

References

Aurora kinase A promotes ovarian tumorigenesis through dysregulation of the cell cycle and suppression of BRCA2.
Yang G, Chang B, Yang F, Guo X, Cai KQ, Xiao XS, Wang H, Sen S, Hung MC, Mills GB, Chang S, Multani AS, Mercado-Uribe I, Liu J
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Jun 15;16(12):3171-81

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Activity of ribonucleotide reductase helps determine how cells repair DNA double strand breaks.
Burkhalter MD, Roberts SA, Havener JM, Ramsden DA
DNA repair 2009 Nov 2;8(11):1258-63

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Thoc1 deficiency compromises gene expression necessary for normal testis development in the mouse.
Wang X, Chinnam M, Wang J, Wang Y, Zhang X, Marcon E, Moens P, Goodrich DW
Molecular and cellular biology 2009 May;29(10):2794-803

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Identification of SMARCAL1 as a component of the DNA damage response.
Postow L, Woo EM, Chait BT, Funabiki H
The Journal of biological chemistry 2009 Dec 18;284(51):35951-61

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A synthetic lethal siRNA screen identifying genes mediating sensitivity to a PARP inhibitor.
Turner NC, Lord CJ, Iorns E, Brough R, Swift S, Elliott R, Rayter S, Tutt AN, Ashworth A
The EMBO journal 2008 May 7;27(9):1368-77

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Apoptosis induced by replication inhibitors in Chk1-depleted cells is dependent upon the helicase cofactor Cdc45
R Rodriguez, M E Gagou, M Meuth
Cell Death and Differentiation 2008 Feb;15(5):889-898

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Human SMC5/6 complex promotes sister chromatid homologous recombination by recruiting the SMC1/3 cohesin complex to double-strand breaks.
Potts PR, Porteus MH, Yu H
The EMBO journal 2006 Jul 26;25(14):3377-88

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Viral transport of DNA damage that mimics a stalled replication fork.
Jurvansuu J, Raj K, Stasiak A, Beard P
Journal of virology 2005 Jan;79(1):569-80

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 219, 111, 83, 48, 32, 26, 17

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 37

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells of seminiferus ducts.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.