MA1-23271
antibody from Invitrogen Antibodies
Targeting: RAD51
BRCC5, FANCR, HsRad51, HsT16930, RAD51A, RECA
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [8]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Other assay [2]
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Validation data
Reference
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- Product number
- MA1-23271 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAD51 Monoclonal Antibody (14B4)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- A suggested positive control for this product is T24.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 14B4
- Vial size
- 100 µL
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage.
van Sluis M, McStay B
Genes & development 2015 Jun 1;29(11):1151-63
Genes & development 2015 Jun 1;29(11):1151-63
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAD51 in Hela (lane 1) and T24 (lane 2) cell lysates. Samples were probed with a RAD51 monoclonal antibody (Product # MA1-23271).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RAD51 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAD51 Monoclonal Antibody (14B4) (Product # MA1-23271) at a dilution of 1:500 and a HRP-conjugated anti-mouse IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RAD51 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAD51 Monoclonal Antibody (14B4) (Product # MA1-23271) at a dilution of 1:500 and a HRP-conjugated anti-mouse IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RAD51 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAD51 Monoclonal Antibody (14B4) (Product # MA1-23271) at a dilution of 1:500 and a HRP-conjugated anti-mouset IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RAD51 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAD51 Monoclonal Antibody (14B4) (Product # MA1-23271) at a dilution of 1:500 and a HRP-conjugated anti-mouset IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAD51 was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAD51 Monoclonal Antibody (14B4) (Product # MA1-23271) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of RAD51 was achieved by transfecting HeLa cells with RAD51 specific siRNAs (Silencer® select Product # s11734, s11735). Western blot analysis (Fig. a) was performed using whole cell extracts from the RAD51 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with RAD51 Monoclonal Antibody (14B4) (Product # MA1-23271, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RAD51.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of NIH/3T3 (Lane 1), HeLa (Lane 2), RAW 264.7 (Lane 3), Neuro-2A (Lane 4), Jurkat (Lane 5), Raji (Lane 6) and PANC-1 (Lane 7). The blot was probed with Anti-RAD51 Monoclonal Antibody (Product # MA1-23271, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 40 kDa band corresponding to RAD51 was observed in all the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent staning of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody (Product # MA1-23271). Cells were pre-extracted with CSK buffer before fixation with 4% PFA. RAD51 14B4 was used at 1:1,000 dultion. DAPI was used to counterstain the nucleus. Scale bar, 10 mciro-meter.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RAD51 Monoclonal Antibody (14B4) detects Rad51 protein at nuclear foci by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad51 stained by RAD51 Monoclonal Antibody (14B4) (Product # MA1-23271) diluted at 1:500. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of HeLa cell extract prepared with lysis 180 buffer (40 mM Tris-HCl pH8.0, 180 mM NaCl, 1 mMEDTA, 0.5% NP-40). Samples were immunoprecipitated using a monoclonal RAD51 antibody (Product # MA1-23271) followed by Western blot using RAD51 polyclonal antibody (Product # PA5-27195). Whole cell extract was used as input.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RAD51 Monoclonal Antibody (14B4) (Product # MA1-23271) detects RAD51 in nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. RAD51 Monoclonal Antibody (14B4) (Product # MA1-23271) dilution: 1:200. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL