PA1-4985

antibody from Invitrogen Antibodies

Targeting: RAD51 BRCC5, FANCR, HsRad51, HsT16930, RAD51A, RECA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

PA1-4985

Provider

Invitrogen Antibodies

Product name

RAD51 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Purifed from natural sources

Reactivity

Human, Mouse, Chicken/Avian, Xenopus

Host

Rabbit

Isotype

IgG

Vial size

50 µL

Concentration

Conc. Not Determined

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of endogenous RAD51 in crude extract of Xenopus using RAD51 Polyclonal Antibody (Product # PA1-4985, diluted 1:1,000).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of RAD51 foci: formation induced by DNA damage. Normal human diploid cells were irradiated by X-ray (0.5 Gy) and after 6 hr incubation, the cells were fixed and immunostained by using RAD51 Polyclonal Antibody (Product # PA1-4985) as the primary antibody and Alexa 594 labeled anti-rabbit antibody as the secondary antibody. (Data kindly provided by Prof. K. Suzuki, Medical School of Nagasaki Univ.)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Induction of RAD51 foci formation by X-ray Irradiation. Human osteosarcoma U2OS cells X-ray irradiation before and 2h after were immunostained using RAD51 Polyclonal Antibody (Product # PA1-4985) diluted 1:6,000. Lower panels: Hoechst staining.