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LearnNBP2-32622
antibody from Novus Biologicals
Targeting: RAD51
BRCC5, FANCR, HsRad51, HsT16930, RAD51A, RECA
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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- Product number
- NBP2-32622 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP2-32622, RRID:AB_2721122
- Product name
- Rabbit Polyclonal Rad51 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Rad51 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Intermittent treatment with farnesyltransferase inhibitor and sulforaphane improves cellular homeostasis in Hutchinson-Gilford progeria fibroblasts.
Temsirolimus Partially Rescues the Hutchinson-Gilford Progeria Cellular Phenotype.
Sulforaphane enhances progerin clearance in Hutchinson-Gilford progeria fibroblasts.
Gabriel D, Shafry DD, Gordon LB, Djabali K
Oncotarget 2017 Sep 12;8(39):64809-64826
Oncotarget 2017 Sep 12;8(39):64809-64826
Temsirolimus Partially Rescues the Hutchinson-Gilford Progeria Cellular Phenotype.
Gabriel D, Gordon LB, Djabali K
PloS one 2016;11(12):e0168988
PloS one 2016;11(12):e0168988
Sulforaphane enhances progerin clearance in Hutchinson-Gilford progeria fibroblasts.
Gabriel D, Roedl D, Gordon LB, Djabali K
Aging cell 2015 Feb;14(1):78-91
Aging cell 2015 Feb;14(1):78-91
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad51 Antibody [NBP2-32622] - Analysis in human cell line MOLT-4.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Rad51 Antibody [NBP2-32622] - Staining of human cell line U-251 MG shows localization to nucleoli. Antibody staining is shown in green.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rad51 Antibody [NBP2-32622] - Staining of human colon shows moderate nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rad51 Antibody [NBP2-32622] - Staining of human thymus shows moderate nuclear positivity in cortical and medullary cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rad51 Antibody [NBP2-32622] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rad51 Antibody [NBP2-32622] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rad51 Antibody [NBP2-32622] - Staining of human tonsil shows moderate nuclear positivity in germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rad51 Antibody [NBP2-32622] - Staining in human testis and skeletal muscle tissues.. Corresponding RAD51 RNA-seq data are presented for the same tissues.
