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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- GTX118249 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX118249, RRID:AB_10624798
- Product name
- Rad51 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
Submitted references Survivin contributes to DNA repair by homologous recombination in breast cancer cells.
Véquaud E, Desplanques G, Jézéquel P, Juin P, Barillé-Nion S
Breast cancer research and treatment 2016 Jan;155(1):53-63
Breast cancer research and treatment 2016 Jan;155(1):53-63
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody (GTX118249) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: JurKat 10% SDS PAGE GTX118249 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody (GTX118249) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody (GTX118249) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rad51 antibody detects Rad51 protein at cytoplasm and nucleolus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Rad51 protein stained by Rad51 antibody (GTX118249) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rad51 antibody immunoprecipitates Rad51 protein in IP experiments.IP samples: Jurkat whole cell extractA. 40 £gg Jurkat whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of Rad51 protein by 4 £gg Rad51 antibody (GTX118249)5 % SDS-PAGEThe immunoprecipitated Rad51 protein was detected by Rad51 antibody (GTX118249) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rad51 antibody detects Rad51 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Rad51 antibody (GTX118249) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rad51 antibody detects Rad51 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Rad51 antibody (GTX118249) dilution: 1:500.
