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LearnH00005888-M01
antibody from Abnova Corporation
Targeting: RAD51
BRCC5, FANCR, HsRad51, HsT16930, RAD51A, RECA
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- H00005888-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005888-M01, RRID:AB_425644
- Product name
- RAD51 monoclonal antibody (M01), clone 2E5-E5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant RAD51.
- Antigen sequence
MAMQMQLEANADTSVEEESFGPQPISRLEQCGINA
NDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAK
ADKILAVAERYGLSGSDVLDNVAYARAFNTDHQTQ
LLYQASAMMVESRYALLIVDSATALYRTDYSGRGE
LSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQ
VDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRG
ETRICKIYDSPCLPEAEAMFAINADGVGDAKD- Isotype
- IgG
- Antibody clone number
- 2E5-E5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- RAD51 monoclonal antibody (M01), clone 2E5-E5 Western Blot analysis of RAD51 expression in HeLa ( Cat # L013V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of RAD51 expression in transfected 293T cell line by RAD51 monoclonal antibody (M01), clone 2E5-E5.Lane 1: RAD51 transfected lysate(37 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged RAD51 is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
