H00005888-D01P
antibody from Abnova Corporation
Targeting: RAD51
BRCC5, FANCR, HsRad51, HsT16930, RAD51A, RECA
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Proximity ligation assay [1]
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Validation data
Reference
Comment
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- Product number
- H00005888-D01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005888-D01P, RRID:AB_1579508
- Product name
- RAD51 purified MaxPab rabbit polyclonal antibody (D01P)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human RAD51 protein.
- Antigen sequence
MAMQMQLEANADTSVEEESFGPQPISRLEQCGINA
NDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAK
ADKILAEAAKLVPMGFTTATEFHQRRSEIIQITTG
SKELDKLLQGGIETGSITEMFGEFRTGKTQICHTL
AVTCQLPIDRGGGEGKAMYIDTEGTFRPERLLAVA
ERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAM
MVESRYALLIVDSATALYRTDYSGRGELSARQMHL
ARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFA
ADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIY
DSPCLPEAEAMFAINADGVGDAKD- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of RAD51 expression in transfected 293T cell line (H00005888-T02) by RAD51 MaxPab polyclonal antibody.Lane 1: RAD51 transfected lysate(37.00 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- RAD51 MaxPab rabbit polyclonal antibody. Western Blot analysis of RAD51 expression in IMR-32.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between RAD51 and CASP3. HeLa cells were stained with anti-RAD51 rabbit purified polyclonal 1:1200 and anti-CASP3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)