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LearnH00005888-M02
antibody from Abnova Corporation
Targeting: RAD51
BRCC5, FANCR, HsRad51, HsT16930, RAD51A, RECA
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00005888-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005888-M02, RRID:AB_10555520
- Product name
- RAD51 monoclonal antibody (M02), clone 4C11-B4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant RAD51.
- Antigen sequence
MAMQMQLEANADTSVEEESFGPQPISRLEQCGINA
NDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAK
ADKILAVAERYGLSGSDVLDNVAYARAFNTDHQTQ
LLYQASAMMVESRYALLIVDSATALYRTDYSGRGE
LSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQ
VDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRG
ETRICKIYDSPCLPEAEAMFAINADGVGDAKD- Isotype
- IgG
- Antibody clone number
- 4C11-B4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged RAD51 is 0.1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of RAD51 transfected lysate using anti-RAD51 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RAD51 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
