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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [3]
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- Product number
- LS-C679268 - Provider product page
- Provider
- LSBio
- Product name
- RAD51 / RECA Antibody LS-C679268
- Antibody type
- Polyclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in:Hela whole cell lysate,Jurkat whole cell lysate,NIH/3T3 whole cell lysate,K562 whole cell lysate All Lanes: RAD51 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37,27,32 kDa Observed band size: 37 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating RAD51 in K562 whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of product in K562 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the Secondary antibody (1/50000) Lane 2: product (4µg) + K562 whole cell lysate (500µg) Lane 3: K562 whole cell lysate (20µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating RAD51 in K562 whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of product in K562 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the Secondary antibody (1/50000) Lane 2: product (4µg) + K562 whole cell lysate (500µg) Lane 3: K562 whole cell lysate (20µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating RAD51 in K562 whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of product in K562 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the Secondary antibody (1/50000) Lane 2: product (4µg) + K562 whole cell lysate (500µg) Lane 3: K562 whole cell lysate (20µg)
