Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NB300-613AF488 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Clathrin Heavy Chain 1/CHC17 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. Detects clathrin heavy chain.
- Reactivity
- Human, Mouse, Rat, Bovine, Hamster, Simian
- Host
- Mouse
- Conjugate
- Green dye
- Antigen sequence
Electron microscopy and proteolysis
mapping demonstrate that binding o
ccurs towards the central hub of th
e triskelion, N-terminal to the lig
ht chain binding regions.- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [Alexa Fluor (R) 488] [NB300-613AF488] - A cell surface stain was performed on HeLa cells with Clathrin Heavy Chain 1 antibody NB300-613AF488 (blue) along with a matched isotype control NBP2-27287AF488 (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. (Top Image) An intracellular stain was performed on HeLa cells with Clathrin Heavy Chain 1 antibody NB300-613AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. (Bottom Image)