Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- NB300-613 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-613, RRID:AB_10130252
- Product name
- Mouse Monoclonal Clathrin Heavy Chain 1/CHC17 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Detects clathrin heavy chain.
- Reactivity
- Human, Mouse, Rat, Bovine, Hamster, Simian
- Host
- Mouse
- Antigen sequence
Electron microscopy and proteolysis
mapping demonstrate that binding o
ccurs towards the central hub of th
e triskelion, N-terminal to the lig
ht chain binding regions.- Isotype
- IgG
- Vial size
- 100uL
- Concentration
- 6 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Quantification of the Relative Contributions of Loss-of-function and Gain-of-function Mechanisms in TAR DNA-binding Protein 43 (TDP-43) Proteinopathies.
Varying label density allows artifact-free analysis of membrane-protein nanoclusters.
Dab2 inhibits the cholesterol-dependent activation of JNK by TGF-β.
Differential regulation of Smad3 and of the type II transforming growth factor-β receptor in mitosis: implications for signaling.
Lysine acetylation induced by chronic ethanol consumption impairs dynamin-mediated clathrin-coated vesicle release.
HIP1 exhibits an early recruitment and a late stage function in the maturation of coated pits.
Ethanol selectively impairs clathrin-mediated internalization in polarized hepatic cells.
Cascella R, Capitini C, Fani G, Dobson CM, Cecchi C, Chiti F
The Journal of biological chemistry 2016 Sep 9;291(37):19437-48
The Journal of biological chemistry 2016 Sep 9;291(37):19437-48
Varying label density allows artifact-free analysis of membrane-protein nanoclusters.
Baumgart F, Arnold AM, Leskovar K, Staszek K, Fölser M, Weghuber J, Stockinger H, Schütz GJ
Nature methods 2016 Aug;13(8):661-4
Nature methods 2016 Aug;13(8):661-4
Dab2 inhibits the cholesterol-dependent activation of JNK by TGF-β.
Shapira KE, Hirschhorn T, Barzilay L, Smorodinsky NI, Henis YI, Ehrlich M
Molecular biology of the cell 2014 May;25(10):1620-8
Molecular biology of the cell 2014 May;25(10):1620-8
Differential regulation of Smad3 and of the type II transforming growth factor-β receptor in mitosis: implications for signaling.
Hirschhorn T, Barizilay L, Smorodinsky NI, Ehrlich M
PloS one 2012;7(8):e43459
PloS one 2012;7(8):e43459
Lysine acetylation induced by chronic ethanol consumption impairs dynamin-mediated clathrin-coated vesicle release.
Shepard BD, Tuma DJ, Tuma PL
Hepatology (Baltimore, Md.) 2012 Apr;55(4):1260-70
Hepatology (Baltimore, Md.) 2012 Apr;55(4):1260-70
HIP1 exhibits an early recruitment and a late stage function in the maturation of coated pits.
Gottfried I, Ehrlich M, Ashery U
Cellular and molecular life sciences : CMLS 2009 Sep;66(17):2897-911
Cellular and molecular life sciences : CMLS 2009 Sep;66(17):2897-911
Ethanol selectively impairs clathrin-mediated internalization in polarized hepatic cells.
Fernandez DJ, McVicker BL, Tuma DJ, Tuma PL
Biochemical pharmacology 2009 Sep 15;78(6):648-55
Biochemical pharmacology 2009 Sep 15;78(6):648-55
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of 25 ug of human brain lysates.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Cancer biopsies of deparaffinized Human breast carcinoma tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Normal biopsies of deparaffinized Human colon tissue.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of Alexa Fluor (R) 488 conjugate of NB300-613. A cell surface stain was performed on HeLa cells with Clathrin Heavy Chain 1 antibody NB300-613AF488 (blue) along with a matched isotype control NBP2-27287AF488 (orange). Cells were incubated in an a