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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- NB120-11331 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-11331, RRID:AB_2083183
- Product name
- Mouse Monoclonal Clathrin Heavy Chain 1/CHC17 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. Monoclonal Anti-Clathrin Heavy Chain reacts specifically with the clathrin heavy chain (CHC17), and does not cross-react with the muscle isoform CHC22. The epitope recognized by the antibody resides within amino acids 207-264 of bovine clathrin heavy chains. It is reported to be non-reactive with native clathrin in immunocytochemistry and immunoprecipitation (but some staining is seen in Rat1 cells, following 4% paraformaldehyde-0.5% Triton X-100 fixation).
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Mouse
- Antigen sequence
The epitope recognized by the antib
ody resides within amino acids 207-
264 of bovine CHC.- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 2.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Protein partners of dynamin-1 in the retina.
Grossman GH, Ebke LA, Beight CD, Jang GF, Crabb JW, Hagstrom SA
Visual neuroscience 2013 Jul;30(4):129-39
Visual neuroscience 2013 Jul;30(4):129-39
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Clathrin heavy chain Antibody (TD.1) [NB120-11331] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-Clathrin Heavy Chain Clone: TD.1. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. SH-SY5Y 4. SK-N-SH 5. COS7 6. PC12 7. Neuro-2a
