MA1-41015

antibody from Invitrogen Antibodies

Targeting: TNFRSF11A CD265, FEO, LOH18CR1, PDB2, RANK

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-41015

Provider

Invitrogen Antibodies

Product name

RANK Monoclonal Antibody (9A725), PE

Antibody type

Monoclonal

Antigen

Other

Description

This antibody recognizes an epitope located between amino acid residues 330-427 of human RaNK. Suggested positive control: Raw.

Reactivity

Human, Mouse, Rat, Chicken/Avian

Host

Mouse

Conjugate

Yellow dye

Isotype

IgG

Antibody clone number

9A725

Vial size

100 µL

Concentration

0.68 mg/mL

Storage

4° C, store in dark

References

Submitted references

CaSR Induces Osteoclast Differentiation and Promotes Bone Metastasis in Lung Adenocarcinoma.

Liu L, Fan Y, Chen Z, Zhang Y, Yu J
Frontiers in oncology 2020;10:305

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Chondrocytes-Specific Expression of Osteoprotegerin Modulates Osteoclast Formation in Metaphyseal Bone.

Wang B, Jin H, Shu B, Mira RR, Chen D
Scientific reports 2015 Sep 2;5:13667

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Human polymorphonuclear neutrophils express RANK and are activated by its ligand, RANKL.

Riegel A, Maurer T, Prior B, Stegmaier S, Heppert V, Wagner C, Hänsch GM
European journal of immunology 2012 Apr;42(4):975-81

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Intracellular flow analysis of RANK in RAW cells using 0.5 µg of CD265/RANK monoclonal antibody (Product # MA1-41015).  Green histogram represents isotype control antibody; red represents CD265/RANK monoclonal antibody (Product # MA1-41015).

Conjugate

Yellow dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of RANK in K-562 cells (blue) and a matched isotype control (orange). Samples were incubated in RANK monoclonal antibody (Product # MA1-41015) using a dilution of 1 µg/mL for 30 minutes at room temperature. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Both antibodies were conjugated to Phycoerythrin.

Conjugate

Yellow dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of RANK in RAW cells. Samples were incubated in RANK monoclonal antibody (Product # MA1-41015) using a dilution of 0.5 µg. Green histogram represents isotype control antibody; red represents RANK antibody.

Conjugate

Yellow dye

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Analysis of CaSR effects on OCs. (A) Isolation and identification of OC precursor cells. (B-E) Four groups of OC precursor cells were treated with CM from modified A549 cells co-cultured with macrophages to examine potential OC differentiation induction. Flow cytometry was used to detect expression of RANK-positive OCs. TRAP+ multinucleated cells were counted using 400-fold microscope and TRAP-stained OC area was calculated using ImageJ software (NIH). OC number and osteolytic area size in CaSR overexpression group were significantly higher than those in empty vector and negative control groups ( p < 0.001). The difference between negative and empty vector control groups was not statistically significant ( p > 0.05). (F) Protein expression levels of bone matrix degradation-related enzymes cathepsin K and CTR were detected by western blot after CaSR overexpression and knockdown in A549 cells. ImageJ software (NIH) for image processing was used to verify band intensities as a result of semi-quantitative western blots. Protein expression levels of cathepsin K and CTR were significantly higher in CaSR overexpression group than in empty vector and negative control groups ( p < 0.001). (G) The qPCR was used to detect expression of bone matrix degradation-related enzymes cathepsin K and CTR mRNA levels after CaSR overexpression and knockdown. Cathepsin K and CRT mRNA expression levels were significantly higher in CaSR overexpression group than in empty vector and negative c

Conjugate

Yellow dye