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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [11]
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- Validations
- Flow cytometry [2]
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- Product number
- NB100-56029 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56029, RRID:AB_838763
- Product name
- Mouse Monoclonal RANK/TNFRSF11A Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. In RAW cells, a 97 kDa is observed.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
Submitted references Elevated suppressor of cytokine signaling-1 (SOCS-1): a mechanism for dysregulated osteoclastogenesis in HIV transgenic rats.
c-Src links a RANK/αvβ3 integrin complex to the osteoclast cytoskeleton.
Human polymorphonuclear neutrophils express RANK and are activated by its ligand, RANKL.
An efficient and rapid method for cDNA cloning from difficult templates using codon optimization and SOE-PCR: with human RANK and TIMP2 gene as examples.
Locally administered interferon-γ accelerates lipopolysaccharide-induced osteoclastogenesis independent of immunohistological RANKL upregulation.
Follicle-stimulating hormone promotes RANK expression on human monocytes.
Signal peptide mutations in RANK prevent downstream activation of NF-κB.
Direct inhibition of human RANK+ osteoclast precursors identifies a homeostatic function of IL-1beta.
Evidence that receptor activator of nuclear factor (NF)-kappaB ligand can suppress cell proliferation and induce apoptosis through activation of a NF-kappaB-independent and TRAF6-dependent mechanism.
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines.
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines.
Lafferty MK, Fantry L, Bryant J, Jones O, Hammoud D, Weitzmann MN, Lewis GK, Garzino-Demo A, Reid W
Pathogens and disease 2014 Jun;71(1):81-9
Pathogens and disease 2014 Jun;71(1):81-9
c-Src links a RANK/αvβ3 integrin complex to the osteoclast cytoskeleton.
Izawa T, Zou W, Chappel JC, Ashley JW, Feng X, Teitelbaum SL
Molecular and cellular biology 2012 Jul;32(14):2943-53
Molecular and cellular biology 2012 Jul;32(14):2943-53
Human polymorphonuclear neutrophils express RANK and are activated by its ligand, RANKL.
Riegel A, Maurer T, Prior B, Stegmaier S, Heppert V, Wagner C, Hänsch GM
European journal of immunology 2012 Apr;42(4):975-81
European journal of immunology 2012 Apr;42(4):975-81
An efficient and rapid method for cDNA cloning from difficult templates using codon optimization and SOE-PCR: with human RANK and TIMP2 gene as examples.
Huang G, Wen Q, Gao Q, Zhang F, Bai Y
Biotechnology letters 2011 Oct;33(10):1939-47
Biotechnology letters 2011 Oct;33(10):1939-47
Locally administered interferon-γ accelerates lipopolysaccharide-induced osteoclastogenesis independent of immunohistological RANKL upregulation.
Ayon Haro ER, Ukai T, Yokoyama M, Kishimoto T, Yoshinaga Y, Hara Y
Journal of periodontal research 2011 Jun;46(3):361-73
Journal of periodontal research 2011 Jun;46(3):361-73
Follicle-stimulating hormone promotes RANK expression on human monocytes.
Cannon JG, Kraj B, Sloan G
Cytokine 2011 Feb;53(2):141-4
Cytokine 2011 Feb;53(2):141-4
Signal peptide mutations in RANK prevent downstream activation of NF-κB.
Crockett JC, Mellis DJ, Shennan KI, Duthie A, Greenhorn J, Wilkinson DI, Ralston SH, Helfrich MH, Rogers MJ
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2011 Aug;26(8):1926-38
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2011 Aug;26(8):1926-38
Direct inhibition of human RANK+ osteoclast precursors identifies a homeostatic function of IL-1beta.
Lee B, Kim TH, Jun JB, Yoo DH, Woo JH, Choi SJ, Lee YH, Song GG, Sohn J, Park-Min KH, Ivashkiv LB, Ji JD
Journal of immunology (Baltimore, Md. : 1950) 2010 Nov 15;185(10):5926-34
Journal of immunology (Baltimore, Md. : 1950) 2010 Nov 15;185(10):5926-34
Evidence that receptor activator of nuclear factor (NF)-kappaB ligand can suppress cell proliferation and induce apoptosis through activation of a NF-kappaB-independent and TRAF6-dependent mechanism.
Bharti AC, Takada Y, Shishodia S, Aggarwal BB
The Journal of biological chemistry 2004 Feb 13;279(7):6065-76
The Journal of biological chemistry 2004 Feb 13;279(7):6065-76
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines.
Fiumara P, Snell V, Li Y, Mukhopadhyay A, Younes M, Gillenwater AM, Cabanillas F, Aggarwal BB, Younes A
Blood 2001 Nov 1;98(9):2784-90
Blood 2001 Nov 1;98(9):2784-90
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines.
Fiumara P, Snell V, Li Y, Mukhopadhyay A, Younes M, Gillenwater AM, Cabanillas F, Aggarwal BB, Younes A
Blood 2001 Nov 1;98(9):2784-90
Blood 2001 Nov 1;98(9):2784-90
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [PE] [NB100-56029] - An intracellular stain was performed on K-562 cells with RANK/TNFRSF11A (9A725) antibody NB100-56029 (blue) and a matched isotype control NBP1-97005PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [PE] [NB100-56029] - Intracellular flow analysis of RANK in RAW cells using 0.5 ug of RANK antibody. Green histogram represents isotype control antibody (NB100-56029); red represents RANK antibody.
