Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Other assay [1]
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- Product number
- AF683 - Provider product page
- Provider
- R&D Systems
- Product name
- Human RANK/TNFRSF11A Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human RANK in direct ELISAs and Western blots. In these formats, approximately 30% cross-reactivity with recombinant mouse RANK is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9Y6Q6
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references IL-3 inhibits human osteoclastogenesis and bone resorption through downregulation of c-Fms and diverts the cells to dendritic cell lineage.
Gupta N, Barhanpurkar AP, Tomar GB, Srivastava RK, Kour S, Pote ST, Mishra GC, Wani MR
Journal of immunology (Baltimore, Md. : 1950) 2010 Aug 15;185(4):2261-72
Journal of immunology (Baltimore, Md. : 1950) 2010 Aug 15;185(4):2261-72
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Human RANK/TNFRSF11A Antibody Induces Osteoclast Differentiation in Mouse Splenocytes. Human RANK/TNFRSF11A Antigen Affinity-purified Polyclonal Antibody induces osteoclast differentiation in the presence of 20 ng/mL recombinant mouse M-CSF (Catalog # 416-ML), in a dose-dependent manner, as measured by the tartrate-resistant acid phosphatase (TRAP) solution assay. The ED50 for this effect is typically 4-8 µg/mL.