MAB683

antibody from R&D Systems

Targeting: TNFRSF11A CD265, FEO, LOH18CR1, PDB2, RANK

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB683

Provider

R&D Systems

Product name

Human RANK/TNFRSF11A Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human RANK/TNFRSF11A in ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) 4-1BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLymphotoxin beta R, rhNGF R, rhOPG, rhTAJ, rhTNF RI, rhTNF RII, or recombinant mouse (rm) OX40, and rmRANK is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q9Y6Q6


Isotype

IgG

Antibody clone number

80704

Vial size

500 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Caveolin‑1 enhances RANKL‑induced gastric cancer cell migration.

Wang Y, Song Y, Che X, Zhang L, Wang Q, Zhang X, Qu J, Li Z, Xu L, Zhang Y, Fan Y, Hou K, Liu Y, Qu X
Oncology reports 2018 Sep;40(3):1287-1296

---

Characterization and application of two RANK-specific antibodies with different biological activities.

Chypre M, Seaman J, Cordeiro OG, Willen L, Knoop KA, Buchanan A, Sainson RC, Williams IR, Yagita H, Schneider P, Mueller CG
Immunology letters 2016 Mar;171:5-14

---

Langerhans cell precursors acquire RANK/CD265 in prenatal human skin.

Schöppl A, Botta A, Prior M, Akgün J, Schuster C, Elbe-Bürger A
Acta histochemica 2015 May-Jun;117(4-5):425-30

---

RANKL expression, function, and therapeutic targeting in multiple myeloma and chronic lymphocytic leukemia.

Schmiedel BJ, Scheible CA, Nuebling T, Kopp HG, Wirths S, Azuma M, Schneider P, Jung G, Grosse-Hovest L, Salih HR
Cancer research 2013 Jan 15;73(2):683-94

---

Receptor activator for NF-κB ligand in acute myeloid leukemia: expression, function, and modulation of NK cell immunosurveillance.

Schmiedel BJ, Nuebling T, Steinbacher J, Malinovska A, Wende CM, Azuma M, Schneider P, Grosse-Hovest L, Salih HR
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 15;190(2):821-31

---

Regulatory T cells in the bone marrow microenvironment in patients with prostate cancer.

Zhao E, Wang L, Dai J, Kryczek I, Wei S, Vatan L, Altuwaijri S, Sparwasser T, Wang G, Keller ET, Zou W
Oncoimmunology 2012 Mar 1;1(2):152-161

---

Epidermal receptor activator of NF-kappaB ligand controls Langerhans cells numbers and proliferation.

Barbaroux JB, Beleut M, Brisken C, Mueller CG, Groves RW
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 15;181(2):1103-8

---

Epidermal receptor activator of NF-kappaB ligand controls Langerhans cells numbers and proliferation.

Barbaroux JB, Beleut M, Brisken C, Mueller CG, Groves RW
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 15;181(2):1103-8

---

High expression levels of receptor activator of nuclear factor-kappa B ligand associated with human chronic periodontitis are mainly secreted by CD4+ T lymphocytes.

Vernal R, Dutzan N, Hernández M, Chandía S, Puente J, León R, García L, Del Valle I, Silva A, Gamonal J
Journal of periodontology 2006 Oct;77(10):1772-80

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of RANK/TNFRSF11A in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human RANK/TNFRSF11A Monoclonal Antibody (Catalog # MAB683, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).