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LearnPA5-90692
antibody from Invitrogen Antibodies
Targeting: CASP8AP2
CED-4, FLASH, FLJ11208, KIAA1315, RIP25
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- PA5-90692 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FLASH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: TVGAFIDLTQ DASSEAKSEG NHPALAVEDL GCGVIQVDED NCKEEKAQVA NRPLKCIVEE TYIDLTTESP SSCEVKKDEL KSEPGSNCDN SELPGTLHNS HKKRRNISDL NHPHKKQRKE TDLTNKEKTK KPTQDSCENT EAHQKKASKK KAPPVTKDPS SLKATPGIKD SSAALATSTS LSAKNVIKKK GEIIILWTRN DDREILLECQ KRGPSFKTFA YLAAKLDKNP NQVSERFQQL MKLFEKSKCR; Positive Samples: U-87MG, K-562; Cellular Location: Cytoplasm, Mitochondrion, Nucleus, PML body
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.85 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FLASH in extracts of various cell lines. Samples were incubated with FLASH Polyclonal antibody (Product # PA5-90692) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
