GTX111637

antibody from GeneTex

Targeting: CHRM3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

GTX111637

Provider

GeneTex

Proper citation

GeneTex Cat#GTX111637, RRID:AB_11167520

Product name

mAChR M3 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

Single onabotulinumtoxinA 200U dose improved clinical symptoms but not urothelial dysfunction in neurogenic detrusor overactivity due to spinal cord injury.

Chen SF, Jiang YH, Kuo HC
Journal of the Formosan Medical Association = Taiwan yi zhi 2019 Jan;118(1 Pt 1):125-133

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Cell-Type-Specific Translation Profiling Reveals a Novel Strategy for Treating Fragile X Syndrome.

Thomson SR, Seo SS, Barnes SA, Louros SR, Muscas M, Dando O, Kirby C, Wyllie DJA, Hardingham GE, Kind PC, Osterweil EK
Neuron 2017 Aug 2;95(3):550-563.e5

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Suitability of Nicotinic Acetylcholine Receptor α7 and Muscarinic Acetylcholine Receptor 3 Antibodies for Immune Detection: Evaluation in Murine Skin.

Rommel FR, Raghavan B, Paddenberg R, Kummer W, Tumala S, Lochnit G, Gieler U, Peters EM
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2015 May;63(5):329-39

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE GTX111637 diluted at 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX111637 diluted at 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

mAChR M3 antibody detects mAChR M3 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with mAChR M3 antibody (GTX111637) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

mAChR M3 antibody detects mAChR M3 protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: mAChR M3 protein stained by mAChR M3 antibody (GTX111637) diluted at 1:500.Blue: Hoechst 33342 staining.