Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
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Validation data
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- Product number
- PA5-85322 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHRM3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CHRM3 in whole cell extracts using CHRM3 polyclonal antibody (Product # PA5-85322) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CHRM3 was performed by separating 30 µg of whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CHRM3 Polyclonal Antibody (Product # PA5-85322) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CHRM3 was performed in A431 cells fixed in ice-cold MeOH for 5 min. Green: CHRM3 Polyclonal Antibody (Product # PA5-85322) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CHRM3 was performed in A431 cells fixed in ice-cold MeOH for 5 min. Green: CHRM3 Polyclonal Antibody (Product # PA5-85322) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.