18843-1-AP

antibody from Proteintech Group

Targeting: RAB12

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

18843-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#18843-1-AP, RRID:AB_10603469

Product name

RAB12 antibody

Antibody type

Polyclonal

Description

KD/KO validated RAB12 antibody (Cat. #18843-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IHC, IP, WB,ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

LRRK2 kinase mediates increased GCase activity in microglia in response to IFNγ-induced proinflammatory stimulation.

MacDougall EJ, Chen CX, Deneault E, You Z, Kalaydjian D, Abdian N, Durcan TM, Senkevich K, Gan-Or Z, Fon EA
NPJ Parkinson's disease 2026 Mar 6;12(1)

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Inflammatory signaling differentially changes chromatin accessibility and gene expression of the PD- associated kinase LRRK2 between human and mice.

Beilina A, Park JH, Landeck N, Chia R, Ding J, Reed X, Bailey H, Kim C, Kaganovich A, Acri DJ, Brooks J, Mann A, Zaghloul KA, Marsan E, Raphael Gibbs J, DeCasien A, Cookson MR
Molecular neurodegeneration 2026 Mar 18;21(1)

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Vps35 p. D620N causes Lrrk2 kinase hyperactivity, chronic microglial activation and inflammation.

Deng IB, Bu M, Follett J, Sharp R, Mamais A, Xoi L, Yu F, Rabil G, Wall S, Farrer MJ
bioRxiv : the preprint server for biology 2026 Mar 11;

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Inflammatory signaling differentially changes chromatin accessibility and gene expression of the PD- associated kinase LRRK2 between human and mice.

Beilina A, Park JH, Landeck N, Chia R, Ding J, Reed X, Bailey H, Kim C, Kaganovich A, Acri DJ, Brooks J, Mann A, Zaghloul KA, Marsan E, Gibbs JR, DeCasien A, Cookson MR
bioRxiv : the preprint server for biology 2026 Jan 12;

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Phosphorylation-driven effector switching of Rab7 and Rab12 by the leucine-rich repeat kinase 1 in mast cells.

Omar-Kabha J, Omari S, Gorzalczany Y, Amer-Sarsour F, Kleeblatt E, Fukuda M, Ashkenazi A, Sagi-Eisenberg R
Frontiers in immunology 2025;16:1709196

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Leucine-rich repeat kinase 2 impairs the release sites of Parkinson's disease vulnerable dopamine axons.

Chen C, He Q, Tombesi G, Napier E, Jaconelli M, Moreno-Ramos OA, Serio H, Naaldijk Y, Promes V, Schneeweis A, Quinn K, Nasios C, Greggio E, Kozorovitskiy Y, Arango D, Khan AR, Alessi DR, Dombeck DA, Hilfiker S, Awatramani R, Parisiadou L
bioRxiv : the preprint server for biology 2025 Sep 3;

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Regulation of LRRK2 activity by metabolic stress and heavy metal exposure.

Kentros M, Follett J, Subrahmanian N, Chairmandurai A, Melachroinou K, Re DB, de Cabo R, Chia R, Kluss JH, Beilina A, Mortiboys H, LaVoie MJ, Rideout HJ, Cookson MR, Mamais A
Brain research 2025 Sep 15;1863:149785

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LRRK2 mediates haloperidol-induced changes in indirect pathway striatal projection neurons.

Chen C, Masotti M, Shepard N, Promes V, Tombesi G, Arango D, Manzoni C, Greggio E, Hilfiker S, Kozorovitskiy Y, Parisiadou L
Molecular psychiatry 2025 Oct;30(10):4473-4486

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Evaluating Parkinson's disease biomarkers in substantia nigra following sublethal γ-radiation exposure in a large animal model.

Murphy EK, Perl DP, Day RM, Iacono D
NPJ Parkinson's disease 2025 Oct 3;11(1):286

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LRRK2 kinase-mediated accumulation of lysosome-associated phospho-Rabs in tauopathies and synucleinopathies.

Buck SA, Malankhanova T, Strader S, Ma EB, Yim S, Pratt HW, Ervin J, Lee EB, Wang SJ, Cohen TJ, West AB, Sanders LH
Acta neuropathologica 2025 Oct 23;150(1):44

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Emergent glutamate & dopamine dysfunction in VPS35((D620N)) knock-in mice and rapid reversal by LRRK2 inhibition.

Kamesh A, Kadgien CA, Kuhlmann N, Coady S, Pietrantonio A, Cousineau Y, Khayachi A, Jurado Santos A, Hurley EP, Barron JC, Parsons MP, Milnerwood AJ
NPJ Parkinson's disease 2025 May 3;11(1):106

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Accumulation of LRRK2-associated phospho-Rab12 degenerative lysosomes in tauopathies.

Buck SA, Malankhanova T, Ma EB, Yim S, Pratt HW, Ervin J, Wang SJ, Cohen TJ, West AB, Sanders LH
bioRxiv : the preprint server for biology 2025 Jun 9;

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The role of RAB12 in inhibiting osteogenic differentiation and driving metabolic dysregulation in osteoporosis.

Ji P, Li Q, Zhang Y, Jin J, Zhang Y, Yuan Z, Shen G, Cao Q, Wu Y, Wang P, Liu W
Life sciences 2025 Jun 1;370:123590

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Rebalance of mitophagy by inhibiting LRRK2 improves colon alterations in an MPTP in vivo model.

Filippone A, Mannino D, Cucinotta L, Calapai F, Crupi L, Paterniti I, Esposito E
iScience 2024 Oct 18;27(10):110980

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Macrophage LRRK2 hyperactivity impairs autophagy and induces Paneth cell dysfunction.

Sun S, Hodel M, Wang X, De Vicente J, Haritunians T, Debebe A, Hung CT, Ma C, Malique A, Nguyen HN, Agam M, Maloney MT, Goo MS, Kluss JH, Mishra R, Frein J, Foster A, Ballentine S, Pandey U, Kern J, Yang S, Mengesha E, Balasubramanian I, Arguello A, Estrada AA, Gao N, Peter I, McGovern DPB, Henry AG, Stappenbeck TS, Liu TC
Science immunology 2024 Nov 8;9(101):eadi7907

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LRRK2 mediates haloperidol-induced changes in indirect pathway striatal projection neurons.

Chen C, Masotti M, Shepard N, Promes V, Tombesi G, Arango D, Manzoni C, Greggio E, Hilfiker S, Kozorovitskiy Y, Parisiadou L
bioRxiv : the preprint server for biology 2024 Jun 8;

---

Reduced Retinal Pigment Epithelial Autophagy Due to Loss of Rab12 Prenylation in a Human iPSC-RPE Model of Choroideremia.

Raeker MÖ, Perera ND, Karoukis AJ, Chen L, Feathers KL, Ali RR, Thompson DA, Fahim AT
Cells 2024 Jun 19;13(12)

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Low-dose radiation decreases Lrrk2 levels in the striatum of large mammalian brains: New venues to treat Parkinson's disease?

Iacono D, Murphy EK, Stimpson CD, Perl DP, Day RM
Parkinsonism & related disorders 2024 Jul;124:107024

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Adult-onset deletion of ATP13A2 in mice induces progressive nigrostriatal pathway dopaminergic degeneration and lysosomal abnormalities.

Erb ML, Sipple K, Levine N, Chen X, Moore DJ
NPJ Parkinson's disease 2024 Jul 20;10(1):133

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A potential patient stratification biomarker for Parkinson´s disease based on LRRK2 kinase-mediated centrosomal alterations in peripheral blood-derived cells.

Naaldijk Y, Fernández B, Fasiczka R, Fdez E, Leghay C, Croitoru I, Kwok JB, Boulesnane Y, Vizeneux A, Mutez E, Calvez C, Destée A, Taymans JM, Aragon AV, Yarza AB, Padmanabhan S, Delgado M, Alcalay RN, Chatterton Z, Dzamko N, Halliday G, Ruiz-Martínez J, Chartier-Harlin MC, Hilfiker S
NPJ Parkinson's disease 2024 Jan 8;10(1):12

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Mitochondrial dysfunction and mitophagy defects in LRRK2-R1441C Parkinson's disease models.

Williamson MG, Madureira M, McGuinness W, Heon-Roberts R, Mock ED, Naidoo K, Cramb KML, Caiazza MC, Malpartida AB, Lavelle M, Savory K, Humble SW, Patterson R, Davis JB, Connor-Robson N, Ryan BJ, Wade-Martins R
Human molecular genetics 2023 Sep 5;32(18):2808-2821

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Genome-wide screen reveals Rab12 GTPase as a critical activator of Parkinson's disease-linked LRRK2 kinase.

Dhekne HS, Tonelli F, Yeshaw WM, Chiang CY, Limouse C, Jaimon E, Purlyte E, Alessi DR, Pfeffer SR
eLife 2023 Oct 24;12

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Rab12 is a regulator of LRRK2 and its activation by damaged lysosomes.

Wang X, Bondar VV, Davis OB, Maloney MT, Agam M, Chin MY, Cheuk-Nga Ho A, Ghosh R, Leto DE, Joy D, Calvert MEK, Lewcock JW, Di Paolo G, Thorne RG, Sweeney ZK, Henry AG
eLife 2023 Oct 24;12

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Directing LRRK2 to membranes of the endolysosomal pathway triggers RAB phosphorylation and JIP4 recruitment.

Kluss JH, Bonet-Ponce L, Lewis PA, Cookson MR
Neurobiology of disease 2022 Aug;170:105769

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Rab12 Promotes Radioresistance of HPV-Positive Cervical Cancer Cells by Increasing G2/M Arrest.

Huang Y, Tian Y, Zhang W, Liu R, Zhang W
Frontiers in oncology 2021;11:586771

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Impact of Type II LRRK2 inhibitors on signaling and mitophagy.

Tasegian A, Singh F, Ganley IG, Reith AD, Alessi DR
The Biochemical journal 2021 Oct 15;478(19):3555-3573

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Increased LRRK2 kinase activity alters neuronal autophagy by disrupting the axonal transport of autophagosomes.

Boecker CA, Goldsmith J, Dou D, Cajka GG, Holzbaur ELF
Current biology : CB 2021 May 24;31(10):2140-2154.e6

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Biochemical and structural insights into Rab12 interactions with RILP and its family members.

Omar J, Rosenbaum E, Efergan A, Sneineh BA, Yeheskel A, Maruta Y, Fukuda M, Sagi-Eisenberg R
Scientific reports 2021 May 13;11(1):10317

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The Parkinson's disease VPS35[D620N] mutation enhances LRRK2-mediated Rab protein phosphorylation in mouse and human.

Mir R, Tonelli F, Lis P, Macartney T, Polinski NK, Martinez TN, Chou MY, Howden AJM, König T, Hotzy C, Milenkovic I, Brücke T, Zimprich A, Sammler E, Alessi DR
The Biochemical journal 2018 Jun 6;475(11):1861-1883

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Rab12 Regulates Retrograde Transport of Mast Cell Secretory Granules by Interacting with the RILP-Dynein Complex.

Efergan A, Azouz NP, Klein O, Noguchi K, Rothenberg ME, Fukuda M, Sagi-Eisenberg R
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 1;196(3):1091-101

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Phosphorylation of the exchange factor DENND3 by ULK in response to starvation activates Rab12 and induces autophagy.

Xu J, Fotouhi M, McPherson PS
EMBO reports 2015 Jun;16(6):709-18

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Rab12 localizes to Shiga toxin-induced plasma membrane invaginations and controls toxin transport.

Rydell GE, Renard HF, Garcia-Castillo MD, Dingli F, Loew D, Lamaze C, Römer W, Johannes L
Traffic (Copenhagen, Denmark) 2014 Jul;15(7):772-87

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 18843-1-AP(RAB12 antibody) at dilution of 1:500

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver using 18843-1-AP(RAB12 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The RAB12 antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human RAB12. This antibody recognizes human, mouse, rat antigen. The RAB12 antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

18843-1-AP.pdf

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