Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF5038 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse TIAM1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects endogenous human and mouse TIAM1 in Western blots.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q13009
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references TIAM1 Antagonizes TAZ/YAP Both in the Destruction Complex in the Cytoplasm and in the Nucleus to Inhibit Invasion of Intestinal Epithelial Cells.
PTP1B inhibitor promotes endothelial cell motility by activating the DOCK180/Rac1 pathway.
Diamantopoulou Z, White G, Fadlullah MZH, Dreger M, Pickering K, Maltas J, Ashton G, MacLeod R, Baillie GS, Kouskoff V, Lacaud G, Murray GI, Sansom OJ, Hurlstone AFL, Malliri A
Cancer cell 2017 May 8;31(5):621-634.e6
Cancer cell 2017 May 8;31(5):621-634.e6
PTP1B inhibitor promotes endothelial cell motility by activating the DOCK180/Rac1 pathway.
Wang Y, Yan F, Ye Q, Wu X, Jiang F
Scientific reports 2016 Apr 7;6:24111
Scientific reports 2016 Apr 7;6:24111
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse TIAM1 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, HCT-116 human colorectal carcinoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse TIAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5038) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TIAM1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.