ABIN953349

antibody from antibodies-online

Targeting: MCF2 ARHGEF21, DBL

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN953349

Provider

antibodies-online

Product name

anti-MCF.2 Cell Line Derived Transforming Sequence (MCF2) (AA 803-833), (C-Term) antibody

Antibody type

Polyclonal

Description

Protein A column, followed by peptide affinity purification

Reactivity

Human

Host

Rabbit

Epitope

AA 803-833, C-Term

Vial size

0.4 mL

Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

The suppressor of metastasis Nm23-H1 interacts with the Cdc42 Rho family member and the pleckstrin homology domain of oncoprotein Dbl-1 to suppress cell migration.

Murakami M, Meneses PI, Lan K, Robertson ES
Cancer biology & therapy 2008 May;7(5):677-88

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Galphaq directly activates p63RhoGEF and Trio via a conserved extension of the Dbl homology-associated pleckstrin homology domain.

Rojas RJ, Yohe ME, Gershburg S, Kawano T, Kozasa T, Sondek J
The Journal of biological chemistry 2007 Oct 5;282(40):29201-10

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Activated ezrin promotes cell migration through recruitment of the GEF Dbl to lipid rafts and preferential downstream activation of Cdc42.

Prag S, Parsons M, Keppler MD, Ameer-Beg SM, Barber P, Hunt J, Beavil AJ, Calvert R, Arpin M, Vojnovic B, Ng T
Molecular biology of the cell 2007 Aug;18(8):2935-48

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

WB

Immunohistochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC

Flow cytometry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

FACS