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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-8986 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-8986, RRID:AB_2116746
- Product name
- Anti-HNF1A
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references c-Myc is required for the CHREBP-dependent activation of glucose-responsive genes.
Hepatocyte nuclear factor 1 is essential for transcription of sodium-dependent vitamin C transporter protein 1.
Interaction of intestinal and pancreatic transcription factors in the regulation of CFTR gene expression.
Intronic enhancers coordinate epithelial-specific looping of the active CFTR locus.
Functional targets of the monogenic diabetes transcription factors HNF-1alpha and HNF-4alpha are highly conserved between mice and humans.
Specific expression and regulation of hepassocin in the liver and down-regulation of the correlation of HNF1alpha with decreased levels of hepassocin in human hepatocellular carcinoma.
Hnf1alpha (MODY3) controls tissue-specific transcriptional programs and exerts opposed effects on cell growth in pancreatic islets and liver.
Hepatocyte nuclear factor-1alpha regulates glucocorticoid receptor expression to control postnatal body growth.
Proteomic screen defines the hepatocyte nuclear factor 1alpha-binding partners and identifies HMGB1 as a new cofactor of HNF1alpha.
Hepatocyte nuclear factor-1 as marker of epithelial phenotype reveals marrow-derived hepatocytes, but not duct cells, after liver injury in mice.
Hepatocyte nuclear factor-4alpha is a central transactivator of the mouse Ntcp gene.
Cholesterol dependent downregulation of mouse and human apical sodium dependent bile acid transporter (ASBT) gene expression: molecular mechanism and physiological consequences.
A Crucial Role of Hepatocyte Nuclear Factor-4 Expression in the Differentiation of Human Ductular Hepatocytes
Zhang P, Metukuri MR, Bindom SM, Prochownik EV, O'Doherty RM, Scott DK
Molecular endocrinology (Baltimore, Md.) 2010 Jun;24(6):1274-86
Molecular endocrinology (Baltimore, Md.) 2010 Jun;24(6):1274-86
Hepatocyte nuclear factor 1 is essential for transcription of sodium-dependent vitamin C transporter protein 1.
Michels AJ, Hagen TM
American journal of physiology. Cell physiology 2009 Nov;297(5):C1220-7
American journal of physiology. Cell physiology 2009 Nov;297(5):C1220-7
Interaction of intestinal and pancreatic transcription factors in the regulation of CFTR gene expression.
McCarthy VA, Ott CJ, Phylactides M, Harris A
Biochimica et biophysica acta 2009 Nov-Dec;1789(11-12):709-18
Biochimica et biophysica acta 2009 Nov-Dec;1789(11-12):709-18
Intronic enhancers coordinate epithelial-specific looping of the active CFTR locus.
Ott CJ, Blackledge NP, Kerschner JL, Leir SH, Crawford GE, Cotton CU, Harris A
Proceedings of the National Academy of Sciences of the United States of America 2009 Nov 24;106(47):19934-9
Proceedings of the National Academy of Sciences of the United States of America 2009 Nov 24;106(47):19934-9
Functional targets of the monogenic diabetes transcription factors HNF-1alpha and HNF-4alpha are highly conserved between mice and humans.
Boj SF, Servitja JM, Martin D, Rios M, Talianidis I, Guigo R, Ferrer J
Diabetes 2009 May;58(5):1245-53
Diabetes 2009 May;58(5):1245-53
Specific expression and regulation of hepassocin in the liver and down-regulation of the correlation of HNF1alpha with decreased levels of hepassocin in human hepatocellular carcinoma.
Yu HT, Yu M, Li CY, Zhan YQ, Xu WX, Li YH, Li W, Wang ZD, Ge CH, Yang XM
The Journal of biological chemistry 2009 May 15;284(20):13335-13347
The Journal of biological chemistry 2009 May 15;284(20):13335-13347
Hnf1alpha (MODY3) controls tissue-specific transcriptional programs and exerts opposed effects on cell growth in pancreatic islets and liver.
Servitja JM, Pignatelli M, Maestro MA, Cardalda C, Boj SF, Lozano J, Blanco E, Lafuente A, McCarthy MI, Sumoy L, Guigó R, Ferrer J
Molecular and cellular biology 2009 Jun;29(11):2945-59
Molecular and cellular biology 2009 Jun;29(11):2945-59
Hepatocyte nuclear factor-1alpha regulates glucocorticoid receptor expression to control postnatal body growth.
Lin WY, Hu YJ, Lee YH
American journal of physiology. Gastrointestinal and liver physiology 2008 Sep;295(3):G542-51
American journal of physiology. Gastrointestinal and liver physiology 2008 Sep;295(3):G542-51
Proteomic screen defines the hepatocyte nuclear factor 1alpha-binding partners and identifies HMGB1 as a new cofactor of HNF1alpha.
Yu M, Wang J, Li W, Yuan YZ, Li CY, Qian XH, Xu WX, Zhan YQ, Yang XM
Nucleic acids research 2008 Mar;36(4):1209-19
Nucleic acids research 2008 Mar;36(4):1209-19
Hepatocyte nuclear factor-1 as marker of epithelial phenotype reveals marrow-derived hepatocytes, but not duct cells, after liver injury in mice.
Swenson ES, Guest I, Ilic Z, Mazzeo-Helgevold M, Lizardi P, Hardiman C, Sell S, Krause DS
Stem cells (Dayton, Ohio) 2008 Jul;26(7):1768-77
Stem cells (Dayton, Ohio) 2008 Jul;26(7):1768-77
Hepatocyte nuclear factor-4alpha is a central transactivator of the mouse Ntcp gene.
Geier A, Martin IV, Dietrich CG, Balasubramaniyan N, Strauch S, Suchy FJ, Gartung C, Trautwein C, Ananthanarayanan M
American journal of physiology. Gastrointestinal and liver physiology 2008 Aug;295(2):G226-33
American journal of physiology. Gastrointestinal and liver physiology 2008 Aug;295(2):G226-33
Cholesterol dependent downregulation of mouse and human apical sodium dependent bile acid transporter (ASBT) gene expression: molecular mechanism and physiological consequences.
Thomas C, Landrier JF, Gaillard D, Grober J, Monnot MC, Athias A, Besnard P
Gut 2006 Sep;55(9):1321-31
Gut 2006 Sep;55(9):1321-31
A Crucial Role of Hepatocyte Nuclear Factor-4 Expression in the Differentiation of Human Ductular Hepatocytes
Tomomi Hakoda, Kazuhide Yamamoto, Ryo Terada, Nobuaki Okano, Noriaki Shimada, Takahiro Suzuki, Motowo Mizuno, Yasushi Shiratori
Laboratory Investigation 2003 Oct;83(10):1395-1402
Laboratory Investigation 2003 Oct;83(10):1395-1402
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 213, 118, 83, 50, 33, 26, 18
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 67
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human kidney shows strong nuclear positivity in cells of tubules.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
