AP7689b

antibody from Abcepta

Targeting: DDR2 NTRKR3, TKT, TYRO10

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7689b

Provider

Abcepta

Proper citation

Abgent Cat#AP7689b, RRID:AB_1967529

Product name

TYRO10 Antibody

Antibody type

Polyclonal

Antigen

Recombinant Protein

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of TYRO10 (arrow) using rabbit polyclonal TYRO10 Antibody (Cat.#AP7689b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDR2 gene (Lane 2) (Origene Technologies).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Western blot analysis of TYRO10 Antibody (Cat.#AP7689b) in Jurkat(lane 1), 293(lane 2) and Hela(lane 3) cell line lysates (35ug/lane). TYRO10 (arrow) was detected using the purified Pab."

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human normal lung with TYRO10 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:10~50

Flow cytometry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Flow cytometric analysis of jurkat cells using TYRO10 Antibody(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50