MA5-15356

antibody from Invitrogen Antibodies

Targeting: DDR2 NTRKR3, TKT, TYRO10

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15356

Provider

Invitrogen Antibodies

Product name

DDR2 Monoclonal Antibody (3B11E4)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15356 targets DDR2 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human samples. The MA5-15356 immunogen is purified recombinant fragment of human DDR2 expressed in E. Coli.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3B11E4

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Cardiac Tissue-like 3D Microenvironment Enhances Route towards Human Fibroblast Direct Reprogramming into Induced Cardiomyocytes by microRNAs.

Paoletti C, Marcello E, Melis ML, Divieto C, Nurzynska D, Chiono V
Cells 2022 Feb 25;11(5)

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Screening for potential targets to reduce stenosis in bioprosthetic heart valves.

Foth R, Shomroni O, Sigler M, Hörer J, Cleuziou J, Paul T, Eildermann K
Scientific reports 2021 Jan 28;11(1):2464

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DDR2 using a DDR2 monoclonal antibody (Product # MA5-15356) against a truncated DDR2 recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DDR2 using a DDR2 monoclonal antibody (Product # MA5-15356) against a truncated DDR2 recombinant protein.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of A549 cells using DDR2 monoclonal antibody (Product # MA5-15356) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissues using DDR2 monoclonal antibody (Product # MA5-15356).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 BM production and characterization in vitro: ( a ) schematic representation of BM production in vitro by AHCFs. Image created with Biorender.com under license. ( b ) Immunofluorescence analysis and quantification of AHCFs expressing vimentin, DDR2 and alpha-SMA after 21 days of culture ( n = 3). Nuclei were counterstained using DAPI. Scale bar: 100 um. ( c , d ) Immunofluorescence analysis of ECM proteins laminin, fibronectin, collagen I and IV before (upper panel, n = 3) and after (lower panel, n = 3) decellularization at 21 days of culture. Nuclei were counterstained using DAPI. Scale bar, 100 um.