Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- BAM1440 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Glut2 Biotinylated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Glut2. Stains mouse Glut2-transfected NS0 cells but not NS0 control transfectants. It also detects Glut2 on mouse insulinoma beta TC-6 cells (2).
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Biotin
- Antigen sequence
P14246
- Isotype
- IgG
- Antibody clone number
- 205115
- Vial size
- 200 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Recognition and killing of human and murine pancreatic beta cells by the NK receptor NKp46.
Gur C, Enk J, Kassem SA, Suissa Y, Magenheim J, Stolovich-Rain M, Nir T, Achdout H, Glaser B, Shapiro J, Naparstek Y, Porgador A, Dor Y, Mandelboim O
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 15;187(6):3096-103
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 15;187(6):3096-103
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Glut2 in beta TC-6 Mouse Cell Line. Glut2 was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Rat Anti-Mouse Glut2 Biotinylated Monoclonal Antibody (Catalog # BAM1440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.