LS-C745375

antibody from LSBio

Targeting: SLC2A2 GLUT2

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C745375

Provider

LSBio

Product name

SLC2A2 / GLUT2 Antibody (C-Terminus) LS-C745375

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Storage

Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry with anti-Glut2 antibody showing Glut2 staining in nucleus and cytoplasm of ductal epithelium and of renal glomeruli in human kidney at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-mouse Glut2 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 1 universal HRP polymer detection system and visualized with 3’3-diamino-benzidine substrate (DAB).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry with anti-Glut2 antibody showing Glut2 staining in nucleus and cytoplasm of ductal epithelium and of renal glomeruli in human kidney at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-mouse Glut2 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 1 universal HRP polymer detection system and visualized with 3’3-diamino-benzidine substrate (DAB).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of Anti-Glut2 Antibody. Tissue: mouse kidney. Antigen retrieval: Heat Induced, slides incubated in sodium citrate buffer for 1hr at 90°C. Primary: Rabbit Anti-Glut2 Antibody at 5µg/mL overnight. Blocking: 2% goat serum in TBST. Secondary: Alexa 488 at 1µg/mL for 2hrs at room temperature.