Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AP0636 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- I°ÙB ÉÇ (S23) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to the N-terminal of Human I°ÙB-ÉÇ.
- Description
- The NF-°ÙB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory I°ÙB proteins .Activation occurs via phosphorylation of I°ÙBÏ« at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-°ÙB . I°ÙBÏ« phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate I°ÙB at these activating sites have been identified.The regulation of I°ÙBÉÇ and I°ÙB÷ is similar to that of I°ÙBÏ«. However, the phosphorylation and ubiquitin-mediated degradation of these proteins occurs with much slower kinetics. IKK phosphorylation of I°ÙBÉÇ occurs at Ser19 and Ser23, while I°ÙB÷ can be phosphorylated at Ser18 and Ser22 .
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of I¦ÊB-¦Â (S23) pAb at 1:500 dilutionLane1:A549 whole cell lysateLane2:NIH-3T3 whole cell lysateLane3:H9C2 whole cell lysate