STJ97539

antibody from St John's Laboratory

Targeting: NFKBIB IKBB, TRIP9

Immunocytochemistry

Antibody data

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Validation data

Product number: STJ97539 - Provider product page
Provider: St John's Laboratory
Product name: Anti-NFKBIB antibody (Full Length) [1F3] (STJ97539)
Antibody type: Monoclonal
Description: Mouse monoclonal antibody anti-Nf-Kappa-B Inhibitor Beta (Full Length) is suitable for use in Immunohistochemistry, Immunofluorescence and Immunocytochemistry research applications.
Reactivity: Human, Mouse, Rat
Host: Mouse
Conjugate: Unconjugated
Antigen sequence: NA
Epitope: NA
Isotype: IgG
Antibody clone number: NA
Vial size: NA
Concentration: NA
Storage: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Handling: NA

NFKBIB protein structure - STJ97539 shown in red.

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of Mouse-testis tissue. 1, I Kappa B Beta monoclonal antibody (1F3) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of Human-liver-cancer tissue. 1, I Kappa B Beta monoclonal antibody (1F3) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Human Kidney Tissue using I Kappa B Beta Mouse mAb diluted at 1:200.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma using I Kappa B Beta (mAb diluted at 1:200.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, I Kappa B Beta monoclonal antibody (1F3) was diluted at 1:200 (4Â°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98Â°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, I Kappa B Beta monoclonal antibody (1F3) was diluted at 1:200 (4Â°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98Â°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA