PA5-40909

antibody from Invitrogen Antibodies

Targeting: NFKBIB IKBB, TRIP9

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-40909

Provider

Invitrogen Antibodies

Product name

IkB beta Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: MLRPNPILAR LLRAHGAPEP EGEDEKSGPC SSSSDSDSGD EGDEYDDIVV Sequence homology: Cow: 91%; Dog: 83%; Guinea Pig: 92%; Horse: 92%; Human: 100%; Mouse: 85%; Rabbit: 77%; Rat: 92%

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Quantitative Analysis of Cellular Proteome Alterations in CDV-Infected Mink Lung Epithelial Cells.

Tong M, Yi L, Sun N, Cheng Y, Cao Z, Wang J, Li S, Lin P, Sun Y, Cheng S
Frontiers in microbiology 2017;8:2564

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 Confirmation of the iTRAQ-MS data by western blotting or real-time RT-PCR. (A) Western blot analysis of NF-kappaB1, RelA, MHC-I, RPS29, and NFkappaBIB in PS-infected and control samples at 12 and 24 hpi. GAPDH was served as internal reference. (B) The intensity ratio of the corresponding bands (infection/mock) was quantified using ImageJ software and normalized against GAPDH. (C) Eight selected differently expression proteins related to NF-kappaB pathway were testified using real-time RT-PCR method. Each gene was performed in three independent experiments. The relative gene expression was calculated using 2- DeltaDelta CT model, representative of n -fold changes in comparison with mock-infected samples. Error bars represent the standard error for triplicate samples. * P < 0.05; ** P < 0.01; *** P < 0.001. The data was analyzed by two-way ANOVA followed by Duncan's test.