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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [8]
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- Product number
- 12660-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IkB beta Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAGVACLGK AADADEWCDS GLGSLGPDAA APGGPGLGAE LGPGLSWAPL VFGYVTEDGD TALHLAVIHQ HEPFLDFLLG FSAGTEYMDL QNDLGQTALH LAAILGETST VEKLYAAGAG LCVAERRGHT ALHLACRVGA HACARALLQP RPRRPREAPD TYLAQGPDRT PDTNHTPVAL YPDSDLEKEE EESEEDWKLQ LEAENYEGHT PLHVAVIHKD VEMVRLLRDA GADLDKPEPT CGRSPLHLAV EAQAADVLEL LLRAGANPAA RMYGGRTPLG SAMLRPNPIL ARLLRAHGAP EPEGEDEKSG PCSSSSDSDS GDEGDEYDDI VVHSSRSQTR LPPTPASKPL PDDPRPV (1-356 aa encoded by BC015528)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 12660-1-AP (IkB beta antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 12660-1-AP (IkB beta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 12660-1-AP (IkB beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 12660-1-AP (IkB beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon tissue slide using 12660-1-AP ( IkB beta antibody at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 12660-1-AP (IkB beta antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human endometrial cancer using 12660-1-AP (IkB beta antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human endometrial cancer using 12660-1-AP (IkB beta antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon tissue slide using 12660-1-AP ( IkB beta antibody at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon tissue slide using 12660-1-AP ( IkB beta antibody at dilution of 1:200 (under 40x lens).
